Composite
Part:BBa_K3110007:Design
Designed by: Mrugank Dake, Amartya Pal Group: iGEM19_IISER_Tirupati (2019-10-14)
Medium Promoter Strong RBS lldD
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1202
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 639
Design Notes
lldD was codon optimized to meet synthesis requirements and to remove the illegal EcoRI site present in the genomic lldD. This part contains:
- Medium Promoter:Part:BBa_J23118
- Strong RBS:Part:BBa_B0034
- lldD:Part:BBa_K3110000
- Double Terminator:Part:BBa_B0015
Source
The whole part was synthesized from Twist Bioscience.
References
- Aguilera, L., Campos, E., Gimenez, R., Badia, J., Aguilar, J., & Baldoma, L. (2008). Dual Role of LldR in Regulation of the lldPRD Operon, Involved in L-Lactate Metabolism in Escherichia coli. Journal of Bacteriology, 190(8), 2997–3005. doi:10.1128/jb.02013-07
- parts.igem.org/Part:BBa_K1847008